The availability of the complete annotated sequence of the Drosophila genome coupled with the invention of a number of elegant genetic techniques has made the fruit fly a more attractive model system than ever before. The NCBS-fly facility managed by Centre for Cellular and Molecular Platforms (C-CAMP) offers a world class microinjection service for the generation of transgenic lines, Screening Platforms, Drosophila strain development and also, maintains a large number of mutant strains and transgenic lines.

So, let us do the work for you while you do your research. For more information click here

Services offered

1. Drosophila Transgenesis:

Drosophila embryo injection work for transgenic production is a labour intensive process and requires technical expertise. This process often diverts attention from the core research project. We are offering Drosophila Transgenesis services at competitive prices. We have range of services from DNA preparation, embryo injection, selection of transformants, mapping the insertions to balancing the insertions. With the state-of-art equipment and the experienced staff we are able to generate transgenics in high-throughput rate. The highly motivated team has successfully generated 5000 transgenics for a collaborative project and few hundreds of transgenics for others.

Currently we are offering following service plans:

• P-element Classic - Injection of DNA into the embryos, raising the survived larvae to adulthood and crossing the G0 flies (>100 individual crosses). Identification and collection of transformants; the collected transformant flies (at least 3 independent lines) are sent to the customer and original vials are maintained in the facility for two weeks as backup.

• P-element Excel - This involves mapping the insertions of the screened transgenic lines followed by the Classic service and balancing the insertions.

• Site-Specific Excel - Injection of DNA into the embryos of PhiC31 integrase & selected attP docking site (click here and choose attP sites), crossing of G0 flies, screening the transformants and balancing the insertions.

• Site-Specific BAC - Injection of BAC clones (>30kb) into the embryos of PhiC31 integrase and docking site (click here and choose attP sites), crossing survived adults screening transformants and balancing the insertions.

• MiMIC - This service includes injecting plasmid into the embryos and screening transformants and balancing the insertions. Clients may have to provide the strains to be used for injections or we can order.

• Molecular cloning - In addition to the above transgenic services we offer molecular cloning services like amplifying and cloning full length cDNAs or genomic clones, tagging with epitopes into insect expression vectors.  

Submitting a Drosophila Transgenic Service request:

To submit a request for Drosophila Transgenic services, please register by clicking here. After registration, please login and click on "My requests" in the menu bar followed by "New request".

The desired service form (P-element or Site-specific) can be filled and submitted to us.

Registered users can submit  a service request by clicking here.

2. Screening Platforms:

Screening arm of fly facility is open to Drosophila research groups to use RNAi, TILLING and chemical compound libraries to screen candidates for any gene of interest. Such requests can be taken up under collaboration.

3. Drosophila new strain development:

Developing a new strain (genotype) or a recombinant by crossing two or more fly lines and submitting stable lines to the clients.

4. Drosophila Stock Maintenance:

With the state-of-the-art facility, we offer Drosophila stock maintenance services.  A dedicated team of stock keepers under the guidance of skilled Fly Stock Curator maintains the fly stocks with high standards of fly husbandry.

Achievements and Collaborations

Highly skilled team at our fly facility has generated >5000 transgenics for DPiM(Drosophila Protein interaction Map) a collaborative project with Harvard Medical School, Boston, USA.

We have successfully generated transgenics for BAC clones of size ranging from 35kb to >80kb size.

Currently we are involved in generating transgenics for the project TransgeneOme in collaboration with MPI-CBG, Dresden, Germany.

Some of our esteemed customers

• National Centre for Biological Sciences, TIFR, Bangalore
• Indian Institute of Science, Bangalore
• Jawaharlal Nehru Centre for Advanced Scientific Research, Bangalore
• IISER-Pune
• Agharkar Research Institute, Pune
• Tata Institute of Fundamental Research, Mumbai
• National Brain Research Centre, Manesar
• Banaras Hindu University, Varanasi
• IISER-Bhopal
• Centre for DNA Finger printing and Diagnosis, Hyderabad
• Trinity College of Dublin, Ireland

Contact

Vinay Vikas K.J

 Email: services[at]ccamp.res.in or  tff[at]ncbs.res.in

Phone: +91-80-2366 6231/2