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Drosophila protein interaction map (DPiM): a paradigm for metazoan protein complex interactions. [Drosophila facility]

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TitleDrosophila protein interaction map (DPiM): a paradigm for metazoan protein complex interactions. [Drosophila facility]
Publication TypeJournal Article
Year of Publication2012
AuthorsGuruharsha KG, Obar RA, Mintseris J, Aishwarya K, Krishnan RT, VijayRaghavan K, Artavanis-Tsakonas S
JournalFly (Austin)
Volume6
Issue4
Pagination246-53
Date Published2012 Oct-Dec
ISSN1933-6942
KeywordsAnimals, Cell Line, Computational Biology, Drosophila melanogaster, Drosophila Proteins, Models, Biological, Protein Interaction Mapping, Protein Interaction Maps
Abstract

Proteins perform essential cellular functions as part of protein complexes, often in conjunction with RNA, DNA, metabolites and other small molecules. The genome encodes thousands of proteins but not all of them are expressed in every cell type; and expressed proteins are not active at all times. Such diversity of protein expression and function accounts for the level of biological intricacy seen in nature. Defining protein-protein interactions in protein complexes, and establishing the when, what and where of potential interactions, is therefore crucial to understanding the cellular function of any protein-especially those that have not been well studied by traditional molecular genetic approaches. We generated a large-scale resource of affinity-tagged expression-ready clones and used co-affinity purification combined with tandem mass-spectrometry to identify protein partners of nearly 5,000 Drosophila melanogaster proteins. The resulting protein complex "map" provided a blueprint of metazoan protein complex organization. Here we describe how the map has provided valuable insights into protein function in addition to generating hundreds of testable hypotheses. We also discuss recent technological advancements that will be critical in addressing the next generation of questions arising from the map.

DOI10.4161/fly.22108
Alternate JournalFly (Austin)
PubMed ID23222005
PubMed Central IDPMC3519659
Grant ListP41 HG003487 / HG / NHGRI NIH HHS / United States
R01 HG003616 / HG / NHGRI NIH HHS / United States
5R01HG003616 / HG / NHGRI NIH HHS / United States
P41HG3487 / HG / NHGRI NIH HHS / United States