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Functional implementation of Drosophila itpr mutants by rat Itpr1. [Drosophila facility]

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TitleFunctional implementation of Drosophila itpr mutants by rat Itpr1. [Drosophila facility]
Publication TypeJournal Article
Year of Publication2012
AuthorsChakraborty S, Hasan G
JournalJ Neurogenet
Volume26
Issue3-4
Pagination328-37
Date Published2012 Sep
ISSN1563-5260
KeywordsAnimals, Animals, Genetically Modified, Calcium, Cells, Cultured, Cytosol, Drosophila, Drosophila Proteins, Flight, Animal, Gene Expression Regulation, Genetic Therapy, Inositol 1,4,5-Trisphosphate Receptors, Larva, Movement Disorders, Mutation, Neurons, Physical Stimulation, Rats, Transcription Factors, Wings, Animal
Abstract

The Drosophila inositol 1,4,5-trisphosphate receptor (IP(3)R) and mammalian type-1 IP(3)Rs have 57-60% sequence similarity and share major domain homology with each other. Mutants in the single Drosophila IP(3)R gene, itpr, and Itpr1 knockout mice both exhibit lethality and defects in motor coordination. Here the authors show that the rat type-1 IP(3)R, which is the major neuronal isoform, when expressed in the pan-neuronal domain in Drosophila, functionally complements Drosophila IP(3)R function at cellular and systemic levels. It rescues the established neuronal phenotypes of itpr mutants in Drosophila, including wing posture, flight, electrophysiological correlates of flight maintenance, and intracellular calcium dynamics. This is the first in vivo demonstration of functional homology between a mammalian and fly IP(3)R. This study also paves the way for cellular and molecular analyses of the spinocerebellar ataxias in Drosophila, since SCA15/16 is known to be caused by heterozygosity of human ITPR1.

DOI10.3109/01677063.2012.697501
Alternate JournalJ. Neurogenet.
PubMed ID22817477