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Light driven ultrafast electron transfer in oxidative redding of Green Fluorescent Proteins. [Protein Technology Facility]

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TitleLight driven ultrafast electron transfer in oxidative redding of Green Fluorescent Proteins. [Protein Technology Facility]
Publication TypeJournal Article
Year of Publication2013
AuthorsSaha R, Verma PKumar, Rakshit S, Saha S, Mayor S, Pal SKumar
JournalSci Rep
Volume3
Pagination1580
Date Published2013
ISSN2045-2322
KeywordsBenzoquinones, Electron Transport, Green Fluorescent Proteins, Light, Oxidation-Reduction
Abstract

Fluorescent proteins undergoing green to red (G/R) photoconversion have proved to be potential tools for investigating dynamic processes in living cells and for photo-localization nanoscopy. However, the photochemical reaction during light induced G/R photoconversion of fluorescent proteins remains unclear. Here we report the direct observation of ultrafast time-resolved electron transfer (ET) during the photoexcitation of the fluorescent proteins EGFP and mEos2 in presence of electron acceptor, p-benzoquinone (BQ). Our results show that in the excited state, the neutral EGFP chromophore accepts electrons from an anionic electron donor, Glu222, and G/R photoconversion is facilitated by ET to nearby electron acceptors. By contrast, mEos2 fails to produce the red emitting state in the presence of BQ; ET depletes the excited state configuration en route to the red-emitting fluorophore. These results show that ultrafast ET plays a pivotal role in multiple photoconversion mechanisms and provide a method to modulate the G/R photoconversion process.

DOI10.1038/srep01580
Alternate JournalSci Rep
PubMed ID23552964
PubMed Central IDPMC3615570