Mechanochemical feedback control of dynamin independent endocytosis modulates membrane tension in adherent cells. [Microfluidics and Microfabrication Facility]

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TitleMechanochemical feedback control of dynamin independent endocytosis modulates membrane tension in adherent cells. [Microfluidics and Microfabrication Facility]
Publication TypeJournal Article
Year of Publication2018
AuthorsThottacherry JJose, Kosmalska AJoanna, Kumar A, Vishen ASingh, Elosegui-Artola A, Pradhan S, Sharma S, Singh PP, Guadamillas MC, Chaudhary N, Vishwakarma R, Trepat X, Del Pozo MA, Parton RG, Rao M, Pullarkat P, Roca-Cusachs P, Mayor S
JournalNat Commun
Volume9
Issue1
Pagination4217
Date Published2018 10 11
ISSN2041-1723
KeywordsAnimals, Biomechanical Phenomena, Cell Adhesion, Cell Membrane, Dynamins, Endocytosis, Feedback, Physiological, Mechanotransduction, Cellular, Mice, Signal Transduction, Temperature, Vinculin
Abstract

Plasma membrane tension regulates many key cellular processes. It is modulated by, and can modulate, membrane trafficking. However, the cellular pathway(s) involved in this interplay is poorly understood. Here we find that, among a number of endocytic processes operating simultaneously at the cell surface, a dynamin independent pathway, the CLIC/GEEC (CG) pathway, is rapidly and specifically upregulated upon a sudden reduction of tension. Moreover, inhibition (activation) of the CG pathway results in lower (higher) membrane tension. However, alteration in membrane tension does not directly modulate CG endocytosis. This requires vinculin, a mechano-transducer recruited to focal adhesion in adherent cells. Vinculin acts by controlling the levels of a key regulator of the CG pathway, GBF1, at the plasma membrane. Thus, the CG pathway directly regulates membrane tension and is in turn controlled via a mechano-chemical feedback inhibition, potentially leading to homeostatic regulation of membrane tension in adherent cells.

DOI10.1038/s41467-018-06738-5
Alternate JournalNat Commun
PubMed ID30310066
PubMed Central IDPMC6181995
Grant List / / Wellcome Trust / United Kingdom