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Method of Preparation of Human Induced Pluripotent Stem Cells (iPSC)

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The Novel Technology:

 
A novel method to prepare iPS cells using an Adeno Associated Virus (AAV) -based vector to reprogram human somatic cells followed by site-specific integration of the reprogramming factor at AAVS 1 locus to generate human iPS cells. Reprogrammed cells can be fully selected using a selection tool and removed from iPSC by Cre-loxP design. Additionally this invention allows for effective removal of AAV vector from the site of integration after reprogramming of the somatic cells.
 
Applications:
  • To study the development and course of different diseases of diseased patients and forming specific cell types (neurons, heart muscle, liver or pancreas cells)
  • To generate integration-free induced pluripotent stem cells by reprogramming somatic cells
  • To make site-specific integration of therapeutic transgenes/Reporter genes in AAVS1 locus on human genome and integration of oncogenic genes to derive iPS cell lines from primary culture cells
  • To discover new drugs or treat medical conditions through cell therapies
  • To test drug efficacies in the generated iPS cells 
Advantages:
  • Precise production of virus-free and integration-free iPS cells
  • More reliable and consistant method
  • Higher yield of iPS cells than compared to existing method
  • Site-specific integration of AAV vector cassette
Business Model:
 
This invention is in early stage of development. Looking for potential licensee(s) interested in taking the technology to next step of development.
 
Principal Investigator(s): Dr. Sanjay Kumar, CMC
 
Invention ID: CMP-030
 
IP status: Patent Pending
 
Jurisdictions: India (IN) 
 
For more details, please write to:
techtransfer @ccamp.res.in