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A protein complex network of Drosophila melanogaster. [Drosophila facility]

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TitleA protein complex network of Drosophila melanogaster. [Drosophila facility]
Publication TypeJournal Article
Year of Publication2011
AuthorsGuruharsha KG, Rual J-F, Zhai B, Mintseris J, Vaidya P, Vaidya N, Beekman C, Wong C, Rhee DY, Cenaj O, McKillip E, Shah S, Stapleton M, Wan KH, Yu C, Parsa B, Carlson JW, Chen X, Kapadia B, VijayRaghavan K, Gygi SP, Celniker SE, Obar RA, Artavanis-Tsakonas S
JournalCell
Volume147
Issue3
Pagination690-703
Date Published2011 Oct 28
ISSN1097-4172
KeywordsAnimals, Drosophila melanogaster, Drosophila Proteins, Proteasome Endopeptidase Complex, Protein Interaction Mapping, Proteomics, SNARE Proteins
Abstract

Determining the composition of protein complexes is an essential step toward understanding the cell as an integrated system. Using coaffinity purification coupled to mass spectrometry analysis, we examined protein associations involving nearly 5,000 individual, FLAG-HA epitope-tagged Drosophila proteins. Stringent analysis of these data, based on a statistical framework designed to define individual protein-protein interactions, led to the generation of a Drosophila protein interaction map (DPiM) encompassing 556 protein complexes. The high quality of the DPiM and its usefulness as a paradigm for metazoan proteomes are apparent from the recovery of many known complexes, significant enrichment for shared functional attributes, and validation in human cells. The DPiM defines potential novel members for several important protein complexes and assigns functional links to 586 protein-coding genes lacking previous experimental annotation. The DPiM represents, to our knowledge, the largest metazoan protein complex map and provides a valuable resource for analysis of protein complex evolution.

DOI10.1016/j.cell.2011.08.047
Alternate JournalCell
PubMed ID22036573
PubMed Central IDPMC3319048
Grant ListR01 HG003616-06 / HG / NHGRI NIH HHS / United States
R01 HG003616-01A1 / HG / NHGRI NIH HHS / United States
R01 HG003616-05 / HG / NHGRI NIH HHS / United States
P41 HG003487-07 / HG / NHGRI NIH HHS / United States
R01 HG003616-02 / HG / NHGRI NIH HHS / United States
P41HG3487 / HG / NHGRI NIH HHS / United States
R01 HG003616-04 / HG / NHGRI NIH HHS / United States
P41 HG003487 / HG / NHGRI NIH HHS / United States
R01 HG003616 / HG / NHGRI NIH HHS / United States
5R01HG003616 / HG / NHGRI NIH HHS / United States
R01 HG003616-03 / HG / NHGRI NIH HHS / United States
R01 HG003616-05S1 / HG / NHGRI NIH HHS / United States