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Unique, polyfucosylated glycan-receptor interactions are essential for regeneration of Hydra magnipapillata.

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TitleUnique, polyfucosylated glycan-receptor interactions are essential for regeneration of Hydra magnipapillata.
Publication TypeJournal Article
Year of Publication2014
AuthorsSahadevan S, Antonopoulos A, Haslam SM, Dell A, Ramaswamy S, Babu P
JournalACS Chem Biol
Volume9
Issue1
Pagination147-55
Date Published2014 Jan 17
ISSN1554-8937
KeywordsAnimals, Carbohydrate Sequence, Hydra, Lectins, C-Type, Molecular Sequence Data, Polysaccharides, Regeneration
Abstract

Cell-cell communications, cell-matrix interactions, and cell migrations play a major role in regeneration. However, little is known about the molecular players involved in these critical events, especially cell surface molecules. Here, we demonstrate the role of specific glycan-receptor interactions in the regenerative process using Hydra magnipapillata as a model system. Global characterization of the N- and O-glycans expressed by H. magnipapillata using ultrasensitive mass spectrometry revealed mainly polyfucosylated LacdiNAc antennary structures. Affinity purification showed that a putative C-type lectin (accession number Q6SIX6) is a likely endogenous receptor for the novel polyfucosylated glycans. Disruption of glycan-receptor interactions led to complete shutdown of the regeneration machinery in live Hydra. A time-dependent, lack-of-regeneration phenotype observed upon incubation with exogenous fuco-lectins suggests the involvement of a polyfucose receptor-mediated signaling mechanism during regeneration. Thus, for the first time, the results presented here provide direct evidence for the role of polyfucosylated glycan-receptor interactions in the regeneration of H. magnipapillata.

DOI10.1021/cb400486t
Alternate JournalACS Chem. Biol.
PubMed ID23972202
Grant ListBB/F008309/1 / / Biotechnology and Biological Sciences Research Council / United Kingdom
/ / Biotechnology and Biological Sciences Research Council / United Kingdom