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A Method to Identify and Isolate Pluripotent Stem Cells Using Endogenous Blue Fluorescence

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The Novel Technology:

            
This invention uses blue autofluorescence of the fat bodies of Human Embryonic Stem cells (hES) and Human Induced Pluripotent Stem cells (hiPS) as an endogenous marker to allow both identification and mechanical isolation of human pluripotent cells. Cells with higher blue auto-fluorescence can also be separated easily and reliably from the non-pluripotent population as single cells.
 
Applications:
  • To use for identifying and isolating human pluripotent stem cells from their differentiated counterparts rapidly and efficiently without modifying the cells
  • To use for both small and large scale cultures equally well
  • To monitor/quantitate/assay efficiency of conversion of various somatic cell types toward pluripotency under different experimental conditions
  • To remove undifferentiated teratoma forming cells
  • To lend itself to high-throughput assays to monitor differentiation/loss of pluripotency and analyse the biochemical process involved
  • To use for isolating and getting both mouse and human pluripotent cells
Advantages:
  • Label –free method to identify human  pluripotent stem cells
  • Useful for High-Throughput identification
  • Single cell isolation possible
  • Can use normal 405 laser and conventional  flow cytometry/microscopy for detection of blue autofluoresence
  • Possible to determine the stage of differentiation e.g.  efficiency of reprogramming
  • Levels of endogenous fluorescence is quantifiable
  • Cost effective method to identify and isolate hiPS, hES and somatic/differentiated cells
Business Model:
 
Proof-of-concept has been demonstrated. Currently, looking for potential licensee(s) interested in taking the technology to next stage of development.
 
Principal Investigator(s): Prof. Mitradas M. Panicker, NCBS
 
Invention ID: CMP-028
 
IP status: Patent Pending
 
Jurisdictions: United States (US), Europe (EP) 
 
For more details, please write to:
techtransfer @ ccamp.res.in