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Method of Multiplexing DNA Sensors, Localizing DNA Sensors and Obtaining FRET Pair

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The Novel Technology:

 
The technology discloses programmable multiple DNA sensors that maps pH change inside the same living cell for the first time. These DNA sensors are non-interfering to the cell and to each other and function autonomously within the cellular environment. These DNA nanomachines are programmed suitably depending on the relevant organelle such that they function as Fluorescent Resonance Energy Transfer (FRET) based pH sensors. 
 
Applications:
  • To simultaneously map pH in two different organelles namely Golgi and Endosome. 
  • To study cellular pathways and trafficking events in cellulo by monitoring the pH gradients of each pathway
  • To study cellular events that may be linked between two organelles
Advantages:
  • Simultaneous track of two proteins and their distinct environments by using two DNA sensors
  • Enables mapping of intersecting endocytic pathways
  • Precise positioning of more than one DNA nanodevice within subcellular environments
Business Model:
 
This invention is in early stage of development. Looking for potential licensee(s) interested in taking the technology to the next development stage. 
 
Principal Investigator(s): Dr. Yamuna Krishnan, NCBS
 
Invention ID: CMP- 032
 
IP Status: Patent pending
 
Jurisdictions: PCT filed
 
For more details, please write to:
techtransfer @ ccamp.res.in