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Methods for Producing Recombinant Peptides and Protein from Non-Filamentous Fungi

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Methods for Producing Recombinant Peptides and Protein from Non-Filamentous Fungi and Recombinant Host Cell Thereof

The Novel Technology: 

 
Novel technology describes the method for production of recombinant B-Domain Deleted (BDD) Factor VIII using Pichia pastoris expression system. Further, it describes a process to produce a functional recombinant Factor VIII by expressing separately and reconstituting heavy and light chain using the same system. 
 
Applications:
  • To express heavy and light chain of Factor VIII in Pichia pastoris expression system and purify and reconstitute obtaining therapeutic FVIII 
  • To have independent use of heavy and light chain for other purposes than hemophilia treatment
Advantages:
  • Easy handling and safe
  • Reduced risk of immune rejection through glycosylation mechanism
  • Ability to grow to high cell densities and enhances the production level
  • Facilitates purification and imposes huge advantage for industrial production
  • Secretes low amounts of host proteins makes purification of target protein easier
Business Model: 
 
This invention is in early stage of development. Looking for potential licensee(s) interested in taking the technology to next step of development. 
 
Principal Investigator(s): Prof. M. A. Vijiyalakhsmi, CBST
 
Invention ID: CMP-027
 
IP status: Patent Pending
 
Jurisdictions: India (IN), PCT filed
 
For more details, please write to:
techtransfer @ ccamp.res.in