@article {1864,
	title = {Organic mineral supplementation on differential protein profile of Osmanabadi bucks (Capra hircus) [Mass Spectrometry - Proteomics Facility]},
	journal = {Reproductive Biology},
	volume = {21},
	year = {2021},
	month = {07/2021},
	pages = {100533},
	type = {Journal Article},
	abstract = {<p>The present study aimed to determine the differential protein profile of seminal plasma proteins of bucks supplemented with trace minerals. Forty bucks of uniform size and body weight were assigned as ten groups (n = 4). The control group (T1) was fed with the control diet (concentration mixture and roughages) whereas the remaining groups were supplemented the control diet with Zn20 mg (T2), Zn40 mg (T3), Zn60 mg (T4), Cu12.5 mg (T5), Cu25 mg (T6), Cu37.5 mg (T7), Zn20 mg + Cu12.5 mg (T8), Zn40 mg + Cu25 mg (T9), and Zn60 mg + Cu37.5 mg (T10) for eight months. Seminal plasma proteins from each group were subjected to two-dimensional electrophoresis and fifteen differential proteins were selected based on differential expression, subjected to identification using Nano-LC{\textendash}MS/MS (LTQ-Qrbitrap-MS). The identified proteins were Triacylglycerol lipase, EGF like repeats and discoidin domains 3, Lipocalin, Iodothyronine deiodinase, Transcription factor AP2-delta, 60S ribosomal protein L13, IST1 factor associated with ESCRT-III, Lysozyme, Uncharacterized protein (BRI3-binding protein), Uncharacterized protein, Histone deacetylase 11, General transcription factor IIF subunit 2, Nudix hydrolase 6, Protein kinase cAMP-activated catalytic subunit beta and Elongin C. The organic Cu supplemented group is the better than the organic Zn and organic Zn + Cu supplemented groups.</p>
},
	keywords = {Bucks, Differential protein, Fecundity, Organic mineral, Seminal plasma},
	doi = {https://doi.org/10.1016/j.repbio.2021.100533},
	url = {https://www.sciencedirect.com/science/article/abs/pii/S1642431X21000541$\#$},
	author = {Sekar, Backialakshmi and Arangasamy, Arunachalam and Naidu, Sharanya Jeevendra and Reddy, Ippala Janardhan and Bhatta, Raghavendra}
}
@article {1530,
	title = {Elucidating the processes and pathways enriched in buffalo sperm proteome in regulating semen quality [Mass Spectrometry Facility - Proteomics]},
	journal = {Cell Tissue Res},
	year = {2020},
	month = {2020 Nov 05},
	abstract = {<p>Sperm carries a reservoir of proteins regulating the molecular functions to attain functional competence. Semen samples collected from buffalo bulls were assessed for sperm functional attributes (n\&nbsp;=\&nbsp;11) and proteome profiling (n\&nbsp;=\&nbsp;6). Sperm proteins were extracted and profiled by employing LC-MS/MS. Overall, the buffalo sperm contained 1365 proteins, of which 458 were common between the groups. The unique proteins were 477 and 430 in good and poor quality semen, respectively. In the whole proteome of buffalo sperm, sexual reproduction with phosphatidylethanolamine-binding protein1 (PEBP1), fetuin-B (FETUB) and acrosin (ACR) was the most enriched (p\&nbsp;=\&nbsp;8.44E-19) biological process, also with thermogenesis (p\&nbsp;=\&nbsp;0.003), oocyte meiosis (p\&nbsp;=\&nbsp;0.007) and vascular smooth muscle contraction (p\&nbsp;=\&nbsp;0.009) apart from metabolic pathways. In good quality semen, mesenchyme migration (p\&nbsp;=\&nbsp;1.24E-07) and morphogenesis (p\&nbsp;=\&nbsp;0.001) were abundant biological processes. In good quality semen, the fluid shear stress (p\&nbsp;=\&nbsp;0.01) and, in poor quality semen, valine, leucine and isoleucine degradation (p\&nbsp;=\&nbsp;3.8E-05) pathways were enriched. In good quality semen, 7 proteins were significantly (p\&nbsp;\&lt;\&nbsp;0.05) upregulated and 33 proteins were significantly (p\&nbsp;\&lt;\&nbsp;0.05) downregulated. On validating the abundantly expressed sperm proteins, serine protease inhibitor Kazal-type 2-like (SPINK2; 2.17-fold) and neddylin (NEDD8; 1.13-fold) were upregulated and YBX2 was downregulated (0.41-fold) in good quality semen as compared with poor quality semen (1-fold). The present findings revealed the importance of sperm proteins in oocyte maturation, fertilization process and early embryonic development. The variations in the proteomic composition can be used as potential markers for the selection of breeding bulls.</p>
},
	issn = {1432-0878},
	doi = {10.1007/s00441-020-03303-9},
	author = {Binsila, Bala Krishnan and Archana, Santhanahalli Siddalingappa and Ramya, Laxman and Swathi, Divakar and Selvaraju, Sellappan and Gowda, N K Shivakumar and Pal, Din Taran and Rafay, Abu and Bhatta, Raghavendra}
}
