@article {1178,
	title = {Serotonin is essential for eye regeneration in planaria Schmidtea~mediterranea  [Mass Spectrometry (Metabolomics) and Central Imaging \& Flow Cytometry Facilities (INT)]},
	journal = {FEBS Lett},
	year = {2019},
	month = {2019 Sep 17},
	abstract = {<p>Planaria is an ideal system to study factors involved in regeneration and tissue homeostasis. Little is known about the role of metabolites and small molecules in stem cell maintenance and lineage specification in planarians. Using liquid chromatography and mass spectrometry (LC-MS)-based quantitative metabolomics, we determined the relative levels of metabolites in stem cells, progenitors, and differentiated cells of the planarian Schmidtea\&nbsp;mediterranea. Tryptophan and its metabolic product serotonin are significantly enriched in stem cells and progenitor population. Serotonin biosynthesis in these cells is brought about by a noncanonical enzyme, phenylalanine hydroxylase. Knockdown of Smed-pah leads to complete disappearance of eyes in regenerating planaria, while exogenous supply of serotonin and its precursor rescues the eyeless phenotype. Our results demonstrate a key role for serotonin in eye regeneration.</p>
},
	issn = {1873-3468},
	doi = {10.1002/1873-3468.13607},
	author = {Sarkar, Arunabha and Mukundan, Namita and Sowndarya, Sai and Dubey, Vinay Kumar and Babu, Rosana and Lakshmanan, Vairavan and Rangiah, Kannan and Panicker, Mitradas M and Palakodeti, Dasaradhi and Subramanian, Sabarinath Peruvemba and Subramanian, Ramaswamy}
}
@article {502,
	title = {Sensitive UHPLC-MS/SRM method for quantifying olanzapine metabolites and degradation products from sera},
	journal = {Anal. Methods},
	volume = {6},
	year = {2014},
	pages = {5250-5257},
	abstract = {<p class="rtejustify">In recent years{,} the use of antipsychotics like olanzapine has increased leading to potentially serious adverse metabolic effects. A sensitive method to quantify olanzapine and its metabolites is therefore highly needed. A stable isotope dilution ultrahigh performance liquid chromatography-mass spectrometry/selected reaction monitoring based quantitative assay has been developed for the simultaneous estimation of olanzapine and its metabolites. This method includes the parent drug olanzapine{,} its metabolites (desmethyl olanzapine and olanzapine-N-oxide) and degradation derived piperazinium chloride{,} lactam and cyclic amine impurities. All analytes were well resolved and showed a linear relationship across a large dynamic range (0.017-1.25 ng mL-1) for all olanzapine metabolites except the lactam{,} in which the linear relationship was demonstrated at concentrations five times higher (0.085-6.25 ng mL-1). All analytes had regression coefficients higher than 0.998{,} accuracies between 92-113\% and low coefficients of variation (0.94 to 9.3\%). The recovery for all of the analytes from the sera matrix was 80 to 115\%. This validated method is suitable for quantifying olanzapine and its metabolites from small volumes of sera samples with great sensitivity.</p>
},
	doi = {10.1039/C4AY00450G},
	url = {http://dx.doi.org/10.1039/C4AY00450G},
	author = {Rangiah, Kannan}
}
