@article {1422,
	title = {Structural Characterization of an Exopolysaccharide Isolated from Enterococcus faecalis, and Study on its Antioxidant Activity, and Cytotoxicity Against HeLa Cells [Mass Spectrometry - Glycomics]},
	journal = {Curr Microbiol},
	year = {2020},
	month = {2020 Jul 28},
	abstract = {<p>An exopolysaccharide (EPS-I) having the molecular weight ~ 2.6 {\texttimes} 10\&nbsp;Da, was isolated from a Zinc resistant strain of Enterococcus faecalis from costal area. The exopolysaccharide consists of D-mannose, D-glucose, and L-fucose in molar ratio of 9:4:1. The monosaccharide units in the EPS-1 were determined through chemical (total acid hydrolysis and methylation analysis) and spectroscopic (FTIR and H NMR experiment) analysis. The mannose-rich EPS-1 showed total antioxidant activity (1\&nbsp;mg\&nbsp;mL of EPS-I as functional as approximately to 500 {\textpm} 5.2\&nbsp;{\textmu}M of ascorbic acid) and Fe metal ion chelation activity (EC = 405.6\&nbsp;{\textmu}g\&nbsp;mL) and hydroxyl radical scavenging activity (EC = 219.5\&nbsp;{\textmu}g\&nbsp;mL). The in vitro cytotoxicity experiment of EPS-I against cervical carcinoma cell line, HeLa cells showed strong cytotoxic effect (LC = 267.3\&nbsp;{\textmu}g\&nbsp;mL) and at that concentration, it found almost nontoxic against normal healthy cells (HEK-293).</p>
},
	issn = {1432-0991},
	doi = {10.1007/s00284-020-02130-z},
	author = {Choudhuri, Indranil and Khanra, Kalyani and Pariya, Prasenjit and Maity, Gajendra Nath and Mondal, Soumitra and Pati, Bikas Ranjan and Bhattacharyya, Nandan}
}
