@article {1422, title = {Structural Characterization of an Exopolysaccharide Isolated from Enterococcus faecalis, and Study on its Antioxidant Activity, and Cytotoxicity Against HeLa Cells [Mass Spectrometry - Glycomics]}, journal = {Curr Microbiol}, year = {2020}, month = {2020 Jul 28}, abstract = {

An exopolysaccharide (EPS-I) having the molecular weight ~ 2.6 {\texttimes} 10\ Da, was isolated from a Zinc resistant strain of Enterococcus faecalis from costal area. The exopolysaccharide consists of D-mannose, D-glucose, and L-fucose in molar ratio of 9:4:1. The monosaccharide units in the EPS-1 were determined through chemical (total acid hydrolysis and methylation analysis) and spectroscopic (FTIR and H NMR experiment) analysis. The mannose-rich EPS-1 showed total antioxidant activity (1\ mg\ mL of EPS-I as functional as approximately to 500 {\textpm} 5.2\ {\textmu}M of ascorbic acid) and Fe metal ion chelation activity (EC = 405.6\ {\textmu}g\ mL) and hydroxyl radical scavenging activity (EC = 219.5\ {\textmu}g\ mL). The in vitro cytotoxicity experiment of EPS-I against cervical carcinoma cell line, HeLa cells showed strong cytotoxic effect (LC = 267.3\ {\textmu}g\ mL) and at that concentration, it found almost nontoxic against normal healthy cells (HEK-293).

}, issn = {1432-0991}, doi = {10.1007/s00284-020-02130-z}, author = {Choudhuri, Indranil and Khanra, Kalyani and Pariya, Prasenjit and Maity, Gajendra Nath and Mondal, Soumitra and Pati, Bikas Ranjan and Bhattacharyya, Nandan} }