@article {1864, title = {Organic mineral supplementation on differential protein profile of Osmanabadi bucks (Capra hircus) [Mass Spectrometry - Proteomics Facility]}, journal = {Reproductive Biology}, volume = {21}, year = {2021}, month = {07/2021}, pages = {100533}, type = {Journal Article}, abstract = {

The present study aimed to determine the differential protein profile of seminal plasma proteins of bucks supplemented with trace minerals. Forty bucks of uniform size and body weight were assigned as ten groups (n = 4). The control group (T1) was fed with the control diet (concentration mixture and roughages) whereas the remaining groups were supplemented the control diet with Zn20 mg (T2), Zn40 mg (T3), Zn60 mg (T4), Cu12.5 mg (T5), Cu25 mg (T6), Cu37.5 mg (T7), Zn20 mg + Cu12.5 mg (T8), Zn40 mg + Cu25 mg (T9), and Zn60 mg + Cu37.5 mg (T10) for eight months. Seminal plasma proteins from each group were subjected to two-dimensional electrophoresis and fifteen differential proteins were selected based on differential expression, subjected to identification using Nano-LC{\textendash}MS/MS (LTQ-Qrbitrap-MS). The identified proteins were Triacylglycerol lipase, EGF like repeats and discoidin domains 3, Lipocalin, Iodothyronine deiodinase, Transcription factor AP2-delta, 60S ribosomal protein L13, IST1 factor associated with ESCRT-III, Lysozyme, Uncharacterized protein (BRI3-binding protein), Uncharacterized protein, Histone deacetylase 11, General transcription factor IIF subunit 2, Nudix hydrolase 6, Protein kinase cAMP-activated catalytic subunit beta and Elongin C. The organic Cu supplemented group is the better than the organic Zn and organic Zn + Cu supplemented groups.

}, keywords = {Bucks, Differential protein, Fecundity, Organic mineral, Seminal plasma}, doi = {https://doi.org/10.1016/j.repbio.2021.100533}, url = {https://www.sciencedirect.com/science/article/abs/pii/S1642431X21000541$\#$}, author = {Sekar, Backialakshmi and Arangasamy, Arunachalam and Naidu, Sharanya Jeevendra and Reddy, Ippala Janardhan and Bhatta, Raghavendra} } @article {1274, title = {Ozone enhanced production of potentially useful exopolymers from the cyanobacterium Nostoc muscorum [Mass Spectrometry - Glycomics]}, journal = {Polymer Testing}, volume = {84}, year = {2020}, pages = {106385}, abstract = {

Extracellular polysaccharides (EPS) from Nostoc muscorum, a heterocystous, filamentous cyanobacterium isolated from a jhumland (shifting cultivation) soil of Assam, North-East India, was physico-chemically characterized to find out its potential applications and to improve its production with some stress source like ozone. Using Response Surface Methodology (RSM), EPS production was improved. Accordingly, with magnesium sulfate (MgSO4{\textperiodcentered}7H2O) at 62\ mg\ L-1, Sodium Chloride (NaCl) at 58\ mg\ L-1 and 56\ mg\ L-1 di-potassium hydrogen phosphate (K2HPO4), a yield of 126.73\ μg\ mL-1 of EPS in 12 days was obtained which was four-fold higher than un-optimised control. An important finding of this study is that EPS production could be further enhanced by over 50\% with a mild stress by a strong oxidizing agent ozone (O3). Physico-chemical properties of this Ozone induced EPS was evaluated and found identical to uninduced EPS. EPS was composed of the hexoses- Glucose (14.80\%), Galactose (18.01\%) and Mannose (12.64\%), the pentoses- Arabinose (17.86\%) and Xylose (11.66\%), the deoxyhexose- Fucose (12.53\%) and Rhamnose (12.50\%). Attenuated Total Reflectance-Fourier Transform Infrared Spectroscopy (ATR-FTIR) analysis revealed the presence of the functional groups uronic acid and traces of sulfate group. The Zeta potential analysis revealed that the emulsions were stabilized electrosterically rather than by pure electrostatic repulsion and steric stabilization. EPS at 1\% in hydrocarbons and vegetable oils was observed to be an excellent emulsifier (99\%), with reasonable stability. Rheological study revealed that the EPS (1\%) was a non- Newtonian weak gel, useful for emulsification activity. Unlike petroleum-based emulsifiers now in use, bio-based EPS are renewable, economical and eco-friendly. The physico-chemical characteristics suggest their utility in a wide variety of other applications including bioremediation, manufacture of paints, shear reduction in oil drilling etc.

}, keywords = {Cyanobacteria, Extracellular polysaccharides (EPS), Optimization, Ozone (O), Stress induction}, issn = {0142-9418}, doi = {https://doi.org/10.1016/j.polymertesting.2020.106385}, url = {http://www.sciencedirect.com/science/article/pii/S0142941819320951}, author = {Dharitri Borah and Gayathri Rethinam and Subramanian Gopalakrishnan and Jayashree Rout and Naiyf S. Alharbi and Sulaiman Ali Alharbi and Thajuddin Nooruddin} } @conference {1311, title = {Overcoming Barriers in Commercializing Bio-Tech Innovations in India: A Case of Center for Cellular and Molecular Platforms}, booktitle = {2019 Portland International Conference on Management of Engineering and Technology (PICMET)}, year = {2019}, month = {Aug}, abstract = {

Building capabilities to successfully commercialize biotech research into products or solutions, is paramount but challenging to develop, especially in developing ecosystems or countries. Once accomplished, they can turn-around the socioeconomic condition in such countries and make them self-reliant, at least in the healthcare sector. It may also encourage the incumbent research community to transform their research findings into scientific, entrepreneurial or commercial ventures. However, building such {\textquoteleft}innovation ecosystems{\textquoteright} in developing countries, requires scientific, financial and infrastructural support to overcome their existing barriers. In India, one such government-funded non-profit organization which is trying to overcome the existing barriers and building an ecosystem to encourage biotech innovation and entrepreneurship, is the Centre for Cellular and Molecular Platforms (C-CAMP). Since its inception, it has been able to support more than 90 biotech start-ups in funding, mentorship and incubation. In this paper, we start our discussion by understanding some of the major challenges faced by contemporary biotech organizations in India while commercializing their innovations. Subsequently, we attempt to understand how C-CAMP was conceptualized to overcome some of these barriers and how it evolved over the years, to become a nodal agency for inspiring biotech innovations and entrepreneurship. This case study highlights some of the best practices followed by C-CAMP in managing biotech innovation and commercialization. Top Management Teams in biotech-based academia, industry, government or venture capital funding agencies from any country may find these barriers and best practices worth studying and analyzing.

}, keywords = {biotech innovation, biotech organizations, biotech start-ups funding, biotechnology, Centre for Cellular and Molecular Platforms, commercial ventures, commercialization, entrepreneurial ventures, entrepreneurship, financial management, financial support, government, government-funded nonprofit organization, health care, healthcare sector, incumbent research community, India, infrastructural support, innovation management, mentorship, organisational aspects, product research, scientific support, scientific ventures, socio-economic effects, socioeconomic condition, top management teams, venture capital, venture capital funding agencies}, doi = {10.23919/PICMET.2019.8893754}, author = {G. D. Tikas and T. Saiyed and A. Katte} } @article {1201, title = {Overexpression of native Musa-miR397 enhances plant biomass without compromising abiotic stress tolerance in banana [Mass Spectrometry - Metabolomics Facility].}, journal = {Sci Rep}, volume = {9}, year = {2019}, month = {2019 Nov 11}, pages = {16434}, abstract = {

Plant micro RNAs (miRNAs) control growth, development and stress tolerance but are comparatively unexplored in banana, whose cultivation is threatened by abiotic stress and nutrient deficiencies. In this study, a native Musa-miR397 precursor harboring 11 copper-responsive GTAC motifs in its promoter element was identified from banana genome. Musa-miR397 was significantly upregulated (8-10) fold in banana roots and leaves under copper deficiency, correlating with expression of root copper deficiency marker genes such as Musa-COPT and Musa-FRO2. Correspondingly, target laccases were significantly downregulated (\>-2 fold), indicating miRNA-mediated silencing for Cu salvaging. No significant expression changes in the miR397-laccase module were observed under iron stress. Musa-miR397 was also significantly upregulated (\>2 fold) under ABA, MV and heat treatments but downregulated under NaCl stress, indicating universal stress-responsiveness. Further, Musa-miR397 overexpression in banana significantly increased plant growth by 2-3 fold compared with wild-type but did not compromise tolerance towards Cu deficiency and NaCl stress. RNA-seq of transgenic and wild type plants revealed modulation in expression of 71 genes related to diverse aspects of growth and development, collectively promoting enhanced biomass. Summing up, our results not only portray Musa-miR397 as a candidate for enhancing plant biomass but also highlight it at the crossroads of growth-defense trade-offs.

}, issn = {2045-2322}, doi = {10.1038/s41598-019-52858-3}, author = {Patel, Prashanti and Yadav, Karuna and Srivastava, Ashish Kumar and Suprasanna, Penna and Ganapathi, Thumballi Ramabhatta} }