BACKGROUND

The invention is a cell line with selectively inducible knockdown of STIM1 in human Neural precursor cells (hNPCs), thereby leading to pre-mature differentiation into neuronal cells. The said hNPCs derived from human pluripotent stem cells have been stably transduced with inducible lentiviral constructs for the knockdown of STIM1 thereby changing the gene expression. Induced knockdown of STIM-1 decreases the proliferation of hNPCs and induces premature neurogenesis.
 

GRAPHICAL ABSTRACT

GA

APPLICATIONS

  • Research in neurodegenerative disorders where intracellular calcium signaling is considered causative including Alzheimer’s, Huntington’s, Parkinson’s, and Spinocerebellar ataxias.

  • Drug screening and toxicity testing assay.

  • This assay can be developed into commercial drug testing and toxicity testing for various neurodegenerative diseases through regulatory approvals

 

ADVANTAGES

  • Customized induction of hNPCs from proliferative to differentiation into preferred neuronal cells.

 

IP STATUS

Patented granted (Patent No. 399095)