|Title||Dataset for the combined transcriptome assembly of M. oleifera and functional annotation [Next Gen Genomics Facility]|
|Publication Type||Journal Article|
|Year of Publication||2020|
|Authors||K. Shafi M, Joshi AG, Meenakshi I, Pasha SNaseer, Harini K., Mahita J, Sajeevan RSivarajan, Karpe SD, Ghosh P, Nitish S, Gandhimathi A., Mathew OK, Prasanna SHari, Malini M, Mutt E, Naika M, Ravooru N, Rao RM, Shingate PN, Sukhwal A, Sunitha MS, Upadhyay AK, Vinekar RS, Sowdhamini R|
|Journal||Data in Brief|
|Keywords||Annotation, Enrichment analysis, Gene Expression, Metabolic pathway, Orthology, Transcriptome|
In this paper, we present the data acquired during transcriptome analysis of the plant Moringa oleifera  from five different tissues (root, stem, leaf, flower and seed) by RNA sequencing. A total of 271 million reads were assembled with an N50 of 2094bp. The combined transcriptome was assessed for transcript abundance across five tissues. The protein coding genes identified from the transcripts were annotated and used for orthology analysis. Further, enzymes involved in the biosynthesis of select medicinally important secondary metabolites, vitamins and ion transporters were identified and their expression levels across tissues were examined. The data generated by RNA sequencing has been deposited to NCBI public repository under the accession number PRJNA394193 (https://www.ncbi.nlm.nih.gov/bioproject/PRJNA394193).