Developmentally regulated higher-order chromatin interactions orchestrate B cell fate commitment.

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TitleDevelopmentally regulated higher-order chromatin interactions orchestrate B cell fate commitment.
Publication TypeJournal Article
Year of Publication2017
AuthorsBoya R, Yadavalli ADevi, Nikhat S, Kurukuti S, Palakodeti D, Pongubala JMR
JournalNucleic Acids Res
Date Published2017 Aug 17

Genome organization in 3D nuclear-space is important for regulation of gene expression. However, the alterations of chromatin architecture that impinge on the B cell-fate choice of multi-potent progenitors are still unclear. By integrating in situ Hi-C analyses with epigenetic landscapes and genome-wide expression profiles, we tracked the changes in genome architecture as the cells transit from a progenitor to a committed state. We identified the genomic loci that undergo developmental switch between A and B compartments during B-cell fate determination. Furthermore, although, topologically associating domains (TADs) are stable, a significant number of TADs display structural alterations that are associated with changes in cis-regulatory interaction landscape. Finally, we demonstrate the potential roles for Ebf1 and its downstream factor, Pax5, in chromatin reorganization and transcription regulation. Collectively, our studies provide a general paradigm of the dynamic relationship between chromatin reorganization and lineage-specific gene expression pattern that dictates cell-fate determination.

Alternate JournalNucleic Acids Res.
PubMed ID28977418