Microscope images play a crucial role in the investigations of biological systems. With the great advances in light microscopy driving the multi-dimensional imaging data, image processing and analysis becomes inevitable in modern life science research. By increasing the quantity and complexity of the microscopic (imaging) data, considered as a multidimensional numeric data, the advanced computational tools to analyse the data also increases. To extract the useful information from the image data, it is important to understand the basics of images, methods to process images and more importantly skills to use the software tools.
In this course we would discuss the basics of image properties, bioimage analysis, and efficient methods/tools to use, feature extraction with the complete hands on experience. This course will help the participant to build their optimum image analysis workflow for their own research problem
Topics to be covered
Module 1: Basics of Images/Imaging
Module 2: Image Inspection
Module 3: Intensity measurements & Background Subtraction
Module 4: Thresholding & Image Segmentation
Module 5: Colocalization
Module 6: Basic 3D quantitative image analysis
Module 7: Introduction to CellProfiler
Course Outcome
- Familiarizing with digital images
- Appropriate use of Fiji/ImageJ & CellProfiler
- Correct choice of filtering methods based on the problem
- Correct choice of quantitative measurements based on the requirements
- Building optimized workflow.
Target Audience
Graduate students, Post-docs and Faculty who need to analyse their own image data. Basic knowledge in handling microscopic imaging data. Programming knowledge not required!
Course Fee*
Academia - Rs. 5,000.00 plus GST@18% (Rs. 5,900.00 incl. GST)
Startups - Rs. 7,000.00 plus GST@18% (Rs. 8,260.00 incl. GST)
Industry - Rs. 10,000.00 plus GST@18% (Rs. 11,800.00 incl. GST)
*Fee doesn't include accommodation charges
