Title | A protein complex network of Drosophila melanogaster. [Drosophila facility] |
Publication Type | Journal Article |
Year of Publication | 2011 |
Authors | Guruharsha KG, Rual J-F, Zhai B, Mintseris J, Vaidya P, Vaidya N, Beekman C, Wong C, Rhee DY, Cenaj O, McKillip E, Shah S, Stapleton M, Wan KH, Yu C, Parsa B, Carlson JW, Chen X, Kapadia B, VijayRaghavan K, Gygi SP, Celniker SE, Obar RA, Artavanis-Tsakonas S |
Journal | Cell |
Volume | 147 |
Issue | 3 |
Pagination | 690-703 |
Date Published | 2011 Oct 28 |
ISSN | 1097-4172 |
Keywords | Animals, Drosophila melanogaster, Drosophila Proteins, Proteasome Endopeptidase Complex, Protein Interaction Mapping, Proteomics, SNARE Proteins |
Abstract | Determining the composition of protein complexes is an essential step toward understanding the cell as an integrated system. Using coaffinity purification coupled to mass spectrometry analysis, we examined protein associations involving nearly 5,000 individual, FLAG-HA epitope-tagged Drosophila proteins. Stringent analysis of these data, based on a statistical framework designed to define individual protein-protein interactions, led to the generation of a Drosophila protein interaction map (DPiM) encompassing 556 protein complexes. The high quality of the DPiM and its usefulness as a paradigm for metazoan proteomes are apparent from the recovery of many known complexes, significant enrichment for shared functional attributes, and validation in human cells. The DPiM defines potential novel members for several important protein complexes and assigns functional links to 586 protein-coding genes lacking previous experimental annotation. The DPiM represents, to our knowledge, the largest metazoan protein complex map and provides a valuable resource for analysis of protein complex evolution. |
DOI | 10.1016/j.cell.2011.08.047 |
Alternate Journal | Cell |
PubMed ID | 22036573 |
PubMed Central ID | PMC3319048 |
Grant List | R01 HG003616-06 / HG / NHGRI NIH HHS / United States R01 HG003616-01A1 / HG / NHGRI NIH HHS / United States R01 HG003616-05 / HG / NHGRI NIH HHS / United States P41 HG003487-07 / HG / NHGRI NIH HHS / United States R01 HG003616-02 / HG / NHGRI NIH HHS / United States P41HG3487 / HG / NHGRI NIH HHS / United States R01 HG003616-04 / HG / NHGRI NIH HHS / United States P41 HG003487 / HG / NHGRI NIH HHS / United States R01 HG003616 / HG / NHGRI NIH HHS / United States 5R01HG003616 / HG / NHGRI NIH HHS / United States R01 HG003616-03 / HG / NHGRI NIH HHS / United States R01 HG003616-05S1 / HG / NHGRI NIH HHS / United States |
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