Production and characterization of bioactive peptides from rice beans using Bacillus subtilis [Mass Spectrometry - Proteomics Facility]

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TitleProduction and characterization of bioactive peptides from rice beans using Bacillus subtilis [Mass Spectrometry - Proteomics Facility]
Publication TypeJournal Article
Year of Publication2022
AuthorsPadhi S, Chourasia R, Kumari M, Singh SP, Rai AKumar
JournalBioresource Technology
Pagination126932
Date Published01/2022
Type of ArticleJournal Article
KeywordsACE-inhibitory peptide, Bacillus subtilis, Fermented legume, Molecular docking, Rice bean
Abstract

A bioprocess was developed for production of bioactive peptides on microbial fermentation of rice beans using proteolytic Bacillus subtilis strains. The peptides produced were identified by LC-MS/MS analysis, revealing the presence of many unique peptide sequences to individual hydrolysates. On functional properties prediction, antihypertensive peptides (3.90%) were found to be higher in comparison to other bioactive peptides. Among different strains, B. subtilis KN2B fermented hydrolysate exhibited highest angiotensin converting enzyme (ACE)-inhibitory activity (45.73%). Furthermore, 19 selected peptides, including the common and unique peptides were examined for their affinity towards the binding cavity of ACE using molecular docking. The results showed a common peptide PFPIPFPIPIPLP, and another IPFPPIPFLPPI unique to B. subtilis KN2B fermented hydrolysate exhibited promising binding at the ACE binding site with substantial free binding energy. The process developed can be used for the production of bioactive peptides from rice bean for application in nutraceutical industries.

URLhttps://www.sciencedirect.com/science/article/abs/pii/S0960852422002619
DOI10.1016/j.biortech.2022.126932
PubMed ID35248709